The impact of the conjugation manner of targeting agent and tandem cell-penetrating peptide on the efficacy of mitomycin C liposomes in treating triple-negative breast tumors.
Quick Facts
What This Study Found
Liposomes coated with poly-L-arginine (cell-penetrating peptide) and chondroitin sulfate (CD44 targeting) delivered mitomycin C to triple-negative breast tumors in mice, achieving the strongest tumor suppression with a Ki-67 index of just 6%.
Key Numbers
PLA-CS liposomes: 144 nm, 73% encapsulation; Ki-67 index 6% (vs higher in controls); caspase-3 upregulated 13-64 fold; G1 cell cycle arrest; no liver/kidney metastasis; sustained MMC release over 24h
How They Did This
Liposome synthesis and characterization (size, PDI, zeta potential, encapsulation). In vitro: MTT cytotoxicity, cellular uptake, apoptosis, cell cycle, qRT-PCR for caspases in 4T1 cells. In vivo: orthotopic 4T1 tumors in BALB/c mice.
Why This Research Matters
Triple-negative breast cancer has few treatment options. Combining a tumor-targeting molecule with a cell-penetrating peptide on drug-carrying nanoparticles could improve chemotherapy delivery while reducing side effects.
What This Study Doesn't Tell Us
Mouse tumor model. Single tumor type (4T1). PLA-CS vs CS-PLA order matters for efficacy, adding manufacturing complexity. Long-term safety not assessed.
Trust & Context
- Original Title:
- The impact of the conjugation manner of targeting agent and tandem cell-penetrating peptide on the efficacy of mitomycin C liposomes in treating triple-negative breast tumors.
- Published In:
- International journal of pharmaceutics: X, 10, 100457 (2025)
- Authors:
- Salahi, Mehrnaz, Varshosaz, Jaleh, Rostami, Mahboubeh, Esfahani, Salar Nasr, Hekmat, Arsham, Jahanian-Najafaabadi, Ali, Minayian, Mohsen
- Database ID:
- RPEP-13381
Evidence Hierarchy
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Cite This Study
https://rethinkpeptides.com/research/RPEP-13381APA
Salahi, Mehrnaz; Varshosaz, Jaleh; Rostami, Mahboubeh; Esfahani, Salar Nasr; Hekmat, Arsham; Jahanian-Najafaabadi, Ali; Minayian, Mohsen. (2025). The impact of the conjugation manner of targeting agent and tandem cell-penetrating peptide on the efficacy of mitomycin C liposomes in treating triple-negative breast tumors.. International journal of pharmaceutics: X, 10, 100457. https://doi.org/10.1016/j.ijpx.2025.100457
MLA
Salahi, Mehrnaz, et al. "The impact of the conjugation manner of targeting agent and tandem cell-penetrating peptide on the efficacy of mitomycin C liposomes in treating triple-negative breast tumors.." International journal of pharmaceutics: X, 2025. https://doi.org/10.1016/j.ijpx.2025.100457
RethinkPeptides
RethinkPeptides Research Database. "The impact of the conjugation manner of targeting agent and ..." RPEP-13381. Retrieved from https://rethinkpeptides.com/research/salahi-2025-the-impact-of-the
Access the Original Study
Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.
This study breakdown was produced by the RethinkPeptides research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.