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Research library — page 13

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RPEP-00694 · 2001

Scientists Discovered Lacritin — A Tear Gland Protein That Boosts Tear Secretion

Lacritin is a newly identified secreted glycoprotein that is highly expressed in human lacrimal gland, moderately in salivary glands, and slightly in thyroid, with no detectable expression in more than 50 other tissues examined. Recombinant lacritin enhanced unstimulated (constitutive) secretion from lacrimal acinar cells in culture, promoted ductal cell proliferation, and stimulated signaling through tyrosine phosphorylation and calcium release in corneal epithelial cells. It binds several extracellular matrix proteins including collagen IV, laminin-1, and fibronectin. The lacritin gene maps to chromosome 12q13 and consists of five exons with no alternative splicing.

Sanghi, S; Kumar, R; Lumsden, A; Dickinson, D; Klepeis, V; Trinkaus-Randall, V; Frierson, H F; Laurie, G W ·

RPEP-00695 · 2001

Creating Mice Without Dynorphin Reveals Its Roles in Pain, Reward, and Beyond

Prodynorphin knockout mice were viable and fertile with altered pain and reward responses, providing a definitive genetic tool for studying dynorphin's specific roles across pain, addiction, mood, and immune function.

Sharifi, N; Diehl, N; Yaswen, L; Brennan, M B; Hochgeschwender, U · Animal Study

RPEP-00696 · 2001

BPC-157 Reduces Anxiety in Rats Across Two Different Behavioral Tests

BPC-157 at 10 μg/kg and 10 ng/kg IP produced anxiolytic effects comparable to diazepam in both the shock probe/burying test (reduced defensive burying) and light/dark test (increased light exploration) in rats.

Sikiric, P; Jelovac, N; Jelovac-Gjeldum, A; Dodig, G; Staresinic, M; Anic, T; Zoricic, I; Ferovic, D; Aralica, G; Buljat, G; Prkacin, I; Lovric-Bencic, M; Separovic, J; Seiwerth, S; Rucman, R; Petek, M; Turkovic, B; Ziger, T · Animal Study

RPEP-00697 · 2001

BPC-157 Heals Both Colon and Duodenal Damage From Cysteamine Without Needing Stomach Acid

BPC-157 attenuated cysteamine-induced colon and duodenal lesions, including in gastrectomized rats, demonstrating acid-independent healing and extending its protective effects to the entire gut.

Sikiric, P; Seiwerth, S; Grabarevic, Z; Balen, I; Aralica, G; Gjurasin, M; Komericki, L; Perovic, D; Ziger, T; Anic, T; Prkacin, I; Separovic, J; Stancic-Rokotov, D; Lovric-Bencic, M; Mikus, D; Staresinic, M; Aralica, J; DiBiaggio, N; Simec, Z; Turkovic, B; Rotkvic, I; Mise, S; Rucman, R; Petek, M; Sebecic, B; Ivasovic, Z; Boban-Blagaic, A; Sjekavica, I · Animal Study

RPEP-00699 · 2001

The Growth Hormone Secretagogue Receptor Family: From Orphan Receptor to Ghrelin Discovery

The GHS-R family includes the active GHS-R1a (ghrelin receptor, constitutively active) and the orphan GHS-R1b splice variant, with constitutive activity suggesting baseline signaling roles and inverse agonist therapeutic potential.

Smith, R G; Leonard, R; Bailey, A R; Palyha, O; Feighner, S; Tan, C; Mckee, K K; Pong, S S; Griffin, P; Howard, A · In Vitro

RPEP-00700 · 2001

BPC-157 Heals Lung Damage From Toxic Chemicals — Not Just Gut Injuries

BPC-157 attenuated both intratracheal HCl-induced lung lesions and associated gastric damage in rats, demonstrating cytoprotective effects extending beyond the GI tract to the respiratory system.

Stancic-Rokotov, D; Slobodnjak, Z; Aralica, J; Aralica, G; Perovic, D; Staresinic, M; Gjurasin, M; Anic, T; Zoricic, I; Buljat, G; Prkacin, I; Sikiric, P; Seiwerth, S; Rucman, R; Petek, M; Turkovic, B; Kokic, N; Jagic, V; Boban-Blagaic, A · Animal Study

RPEP-00702 · 2001

Natriuretic Peptides and Programmed Cell Death Markers Together Predict Heart Failure Outcomes

Plasma BNP correlated with soluble Fas (apoptosis marker) in CHF patients, and sFas independently predicted prognosis, establishing a link between natriuretic peptide signaling and cardiac programmed cell death.

Tsutamoto, T; Wada, A; Maeda, K; Mabuchi, N; Hayashi, M; Tsutsui, T; Ohnishi, M; Fujii, M; Matsumoto, T; Yamamoto, T; Takayama, T; Kinoshita, M · Cross Sectional

RPEP-00704 · 2001

How Estrogen Keeps Growth Hormone Secretion Active Throughout Life

Estrogen is the proximate sex steroid sustaining GH secretion across the human lifespan, modulating GHRP sensitivity, somatostatin withdrawal, and GH feedback through specific neuroendocrine mechanisms that decline with menopause.

Veldhuis, J D; Evans, W S; Bowers, C Y; Anderson, S · Review

RPEP-00705 · 2001

First Proof That Ghrelin Increases Appetite and Food Intake in Humans

IV ghrelin infusion in healthy humans increased subjective appetite ratings and food intake significantly, alongside GH stimulation, providing the first proof that ghrelin is a human hunger hormone.

Wren, A M; Seal, L J; Cohen, M A; Brynes, A E; Frost, G S; Murphy, K G; Dhillo, W S; Ghatei, M A; Bloom, S R · RCT

RPEP-00706 · 2001

Blocking Each of the Body's Opioid Peptides Increases Pain Differently

ICV anti-beta-endorphin increased acute phase formalin pain, anti-leu-enkephalin increased late inflammatory phase pain, and anti-dynorphin affected both phases, demonstrating peptide-specific roles in endogenous pain modulation.

Wu, H; Hung, K; Ohsawa, M; Mizoguchi, H; Tseng, L F · Animal Study

RPEP-00708 · 2001

Selank Treats Anxiety by Protecting the Body's Natural Anxiety-Relieving Enkephalins

Anxiety patients showed accelerated enkephalin degradation, and Selank's anxiolytic effect correlated with inhibition of enkephalin-degrading enzymes, preserving endogenous opioid-mediated anxiety relief.

Zozulya, A A; Kost, N V; Yu Sokolov, O; Gabaeva, M V; Grivennikov, I A; Andreeva, L N; Zolotarev, Y A; Ivanov, S V; Andryushchenko, A V; Myasoedov, N F; Smulevich, A B · Clinical Trial

RPEP-00711 · 2002

Ghrelin and Synthetic GH Secretagogues: A Unified View of the System

The ghrelin-GHS system is a multi-functional endocrine network with validated roles in GH regulation, appetite control, adiposity, and cardiovascular function, with therapeutic implications across multiple medical specialties.

Arvat, Emanuela; Broglio, Fabio; Aimaretti, Gianluca; Benso, Andrea; Giordano, Roberta; Deghenghi, Romano; Ghigo, Ezio · Review

RPEP-00712 · 2002

Both Ghrelin and Its Inactive Form Des-Acyl Ghrelin Protect Heart and Blood Vessel Cells From Death

Both acylated ghrelin and des-acyl ghrelin inhibited apoptosis in cardiomyocytes and endothelial cells via ERK1/2 and PI3K/Akt pathways, with des-acyl ghrelin's activity demonstrating GHS-R-independent cardiovascular protection.

Baldanzi, Gianluca; Filigheddu, Nicoletta; Cutrupi, Santina; Catapano, Filomena; Bonissoni, Sara; Fubini, Alberto; Malan, Daniela; Baj, Germano; Granata, Riccarda; Broglio, Fabio; Papotti, Mauro; Surico, Nicola; Bussolino, Federico; Isgaard, Jorgen; Deghenghi, Romano; Sinigaglia, Fabiola; Prat, Maria; Muccioli, Giampiero; Ghigo, Ezio; Graziani, Andrea · In Vitro

RPEP-00713 · 2002

Milk Proteins and Their Peptide Fragments Show Anti-HIV Activity

Native bovine lactoferrin and derived peptides (lactoferricin) inhibited HIV entry into cells, with positively charged proteins/peptides showing the strongest activity, supporting milk-derived compounds as natural antivirals.

Berkhout, Ben; van Wamel, Jeroen L B; Beljaars, Leonie; Meijer, Dirk K F; Visser, Servaas; Floris, René · In Vitro

RPEP-00716 · 2002

Argireline: The Anti-Wrinkle Peptide Designed to Mimic Botox Without the Needles

Argireline (Ac-EEMQRR-NH₂), a synthetic six-amino-acid peptide, reduced wrinkle depth by up to 30% in healthy women volunteers after 30 days of topical application at 10% concentration in an oil-in-water emulsion. The peptide works by mimicking botulinum toxin's mechanism: it inhibits neurotransmitter release by interfering with the SNARE complex — the protein machinery that cells use to release chemical signals at nerve endings. While Argireline's inhibitory potency was similar to botulinum toxin type A, its overall efficacy was lower, as expected for a topical peptide versus an injected neurotoxin. Critically, Argireline showed no oral toxicity in vivo and caused no primary skin irritation even at high doses, establishing it as a non-toxic alternative to botulinum toxin injections for cosmetic wrinkle reduction.

Blanes-Mira, C; Clemente, J; Jodas, G; Gil, A; Fernández-Ballester, G; Ponsati, B; Gutierrez, L; Pérez-Payá, E; Ferrer-Montiel, A · Clinical Trial

RPEP-00718 · 2002

Ghrelin as a Multi-System Metabolic Regulator: Beyond Growth Hormone to Appetite, Fat, and Heart

Ghrelin acts as a multi-system metabolic regulator beyond GH release, with validated roles in appetite, energy balance, gastric motility, pancreatic function, cardiovascular regulation, and immune modulation through widely distributed receptors.

Broglio, Fabio; Arvat, Emanuela; Benso, Andrea; Gottero, Cristina; Prodam, Flavia; Granata, Riccarda; Papotti, Mauro; Muccioli, Giampiero; Deghenghi, Romano; Ghigo, Ezio · Review

RPEP-00719 · 2002

Are Natriuretic Peptides Just Markers or Active Players in Heart Disease?

Natriuretic peptides serve dual roles as sensitive cardiac biomarkers AND active cardiovascular protectors, with evidence supporting both diagnostic utility and therapeutic potential through their vasodilatory, natriuretic, and neurohormonal-suppressive actions.

Clerico, Aldo · Review

RPEP-00720 · 2002

Defensins in the Lungs: How Natural Antimicrobial Peptides Protect Your Airways and Could Become Drugs

The review established several key findings about defensins in lung biology: • Humans produce at least 10 defensin molecules — 6 alpha-defensins and 4 beta-defensins — that are structurally and functionally similar • Defensins kill a broad spectrum of microorganisms while permitting little resistance development • Many defensin peptides can neutralize lipopolysaccharide (LPS) effects on macrophages and reduce proinflammatory cytokine release, providing protection against septic shock • Protegrin-1 (a pig-derived minidefensin) served as a template for iseganan, which was in phase III clinical trials for oral mucositis from chemotherapy • Prospective uses included respiratory pathogen control in cystic fibrosis and prevention of ventilator-associated pneumonia • Major technical hurdle: synthesizing large quantities of complexly folded defensin peptides at scale

Cole, Alexander M; Waring, Alan J ·

RPEP-00724 · 2002

The 'Most Potent Vasoconstrictor' Doesn't Seem to Drive Heart Failure

Urotensin-II plasma levels and its gene expression in heart and blood vessels were unchanged in patients with congestive heart failure compared to healthy controls. The peptide levels did not differ across measurement sites (pulmonary artery, left ventricle, coronary sinus, or arm vein), and even when severe heart failure patients received vasodilator therapy that significantly improved their hemodynamics (cardiac index up 78%, wedge pressure down 55%), urotensin-II levels stayed flat over 24 hours. Gene expression of the urotensin-II precursor in heart tissue and blood vessels was also no different between end-stage heart failure patients and controls with normal heart function.

Dschietzig, Thomas; Bartsch, Cornelia; Pregla, Rainer; Zurbrügg, Heinz Robert; Armbruster, Franz Paul; Richter, Christoph; Laule, Michael; Romeyke, Elfrun; Neubert, Charlotte; Voelter, Wolfgang; Baumann, Gert; Stangl, Karl · Clinical Observational

RPEP-00729 · 2002

GH Secretagogues Actually Inhibit Lung Cancer Cell Growth Through Their Receptors

Synthetic peptidyl GH secretagogues inhibited proliferation of human CALU-1 lung carcinoma cells that express high levels of GHS receptors, demonstrating antiproliferative rather than growth-promoting activity in this cancer type.

Ghè, Corrado; Cassoni, Paola; Catapano, Filomena; Marrocco, Tiziana; Deghenghi, Romano; Ghigo, Ezio; Muccioli, Giampiero; Papotti, Mauro · In Vitro

RPEP-00730 · 2002

New Macrocyclic Drug Templates That Mimic Protein Structures for Antiviral Applications

Novel macrocyclic amino acids that constrain peptides into beta-strand conformations were developed as protease inhibitor templates, showing application in antiviral drug design against HIV and HCV proteases.

Glenn, Matthew P; Pattenden, Leonard K; Reid, Robert C; Tyssen, David P; Tyndall, Joel D A; Birch, Christopher J; Fairlie, David P · In Vitro

RPEP-00731 · 2002

Ghrelin and Its Receptor Are Found Throughout the Human Body, Not Just the Stomach and Brain

Ghrelin mRNA was detected in stomach (highest), intestine, pancreas, kidney, and other tissues, while GHS-R1a and GHS-R1b showed distinct but overlapping tissue distributions across virtually all major human organs.

Gnanapavan, Sharmilee; Kola, Blerina; Bustin, Stephen A; Morris, Damian G; McGee, Patrick; Fairclough, Peter; Bhattacharya, Satya; Carpenter, Robert; Grossman, Ashley B; Korbonits, Márta · In Vitro

RPEP-00744 · 2002

BNP Is Better Than ANP for Monitoring Heart Failure Severity in Outpatients

BNP correlated most strongly with NYHA heart failure classification and treatment response in outpatients, outperforming C-terminal ANP and N-terminal ANP as a monitoring biomarker.

Lee, Shang-Chiun; Stevens, Tracy L; Sandberg, Sharon M; Heublein, Denise M; Nelson, Susan M; Jougasaki, Michihisa; Redfield, Margaret M; Burnett, John C · Cross Sectional

RPEP-00746 · 2002

Thymosin Alpha-1 Speeds Up Immune Recovery and Antibody Production in Immunocompromised Animals

Thymosin alpha-1 accelerated restoration of T-cell-mediated neutralizing antibody responses in immunocompromised hosts, demonstrating faster immune reconstitution through both immunomodulatory and direct intracellular mechanisms.

Li, Chun-lin; Zhang, Ting; Saibara, Toshiji; Nemoto, Yoshihisa; Ono, Masafumi; Akisawa, Naoaki; Iwasaki, Shinji; Maeda, Takashi; Onishi, Saburo · Animal Study

RPEP-00750 · 2002

A Simple Blood Test for BNP Could Diagnose Heart Failure in the Emergency Room With 83% Accuracy

In a prospective study of 1,586 emergency department patients presenting with acute dyspnea, B-type natriuretic peptide (BNP) measured by bedside assay was more accurate than any historical finding, physical examination, or laboratory value for identifying congestive heart failure. At a cutoff of 100 pg/mL, BNP had a diagnostic accuracy of 83.4%. At levels below 50 pg/mL, the negative predictive value was 96% — meaning very low BNP effectively rules out heart failure. Of the patients studied, 47% had heart failure, 49% had non-cardiac causes of dyspnea, and 5% had dyspnea from non-cardiac causes despite a history of left ventricular dysfunction. BNP added significant independent predictive power to other clinical variables in multivariate analysis.

Maisel, Alan S; Krishnaswamy, Padma; Nowak, Richard M; McCord, James; Hollander, Judd E; Duc, Philippe; Omland, Torbjørn; Storrow, Alan B; Abraham, William T; Wu, Alan H B; Clopton, Paul; Steg, Philippe G; Westheim, Arne; Knudsen, Catherine Wold; Perez, Alberto; Kazanegra, Radmila; Herrmann, Howard C; McCullough, Peter A ·

RPEP-00751 · 2002

Thymosin Alpha-1 Prevents Breast Cancer Development in Rats

Thymosin alpha-1 significantly inhibited NMU-induced mammary carcinogenesis in Fisher rats, reducing both tumor incidence and multiplicity, demonstrating cancer prevention through immune enhancement.

Moody, Terry W; Tuthill, Cynthia; Badamchian, Mahnaz; Goldstein, Allan L · Animal Study