Fermented Milk Yields Three Blood-Pressure-Lowering Peptides with Proven ACE-Blocking Power

Three short peptides (WRP, WSR, YRP) isolated from milk fermented with Lactobacillus delbrueckii effectively inhibit the blood-pressure-raising enzyme ACE in lab tests.

Wu, Nan et al.·Food & function·2024·Preliminary Evidencein vitro
RPEP-09547In vitroPreliminary Evidence2024RETHINKTHC RESEARCH DATABASErethinkthc.com/research

Quick Facts

Study Type
in vitro
Evidence
Preliminary Evidence
Sample
N=N/A (in vitro)
Participants
In vitro enzyme assay and molecular dynamics simulation

What This Study Found

Three ACE-inhibiting tripeptides (WRP IC50 46.7 μM, YRP IC50 89.6 μM, WSR IC50 300.1 μM) were identified from fermented milk, with molecular dynamics confirming stable enzyme binding for WRP.

Key Numbers

WRP: IC50 46.707 μM. WSR: IC50 300.121 μM. YRP: IC50 89.555 μM. 16 stable tripeptides initially screened from fermented milk proteome.

How They Did This

Proteomics identified peptides in fermented milk before and after ultra-high pressure treatment. Sixteen stable tripeptides were screened via activity prediction, PeptideCutter analysis, and hydrophobicity. ACE inhibition was tested in vitro; molecular dynamics simulation confirmed binding stability.

Why This Research Matters

Rather than just observing that fermented dairy lowers blood pressure, this study pinpoints the specific peptides responsible and maps exactly how they interact with ACE, opening the door to targeted functional food development.

The Bigger Picture

ACE inhibitor drugs (like lisinopril) are a cornerstone of blood pressure management but come with side effects. Food-derived peptides that naturally inhibit the same enzyme could complement or partially replace pharmaceuticals for mild hypertension, especially through everyday dietary choices like fermented dairy.

What This Study Doesn't Tell Us

This is an in vitro and computational study — it does not prove these peptides lower blood pressure in living organisms. Real-world bioavailability after full human digestion remains unconfirmed, and the peptide concentrations achievable through normal dairy consumption are unknown.

Questions This Raises

  • ?Can WRP and YRP survive full human digestion and reach the bloodstream at meaningful concentrations?
  • ?What dose of fermented milk would be needed to deliver a clinically relevant amount of these ACE-inhibitory peptides?
  • ?Do these peptides have any off-target effects beyond ACE inhibition?

Trust & Context

Key Stat:
IC50 46.7 μM WRP tripeptide's ACE inhibitory potency — the most active of three peptides found in fermented milk
Evidence Grade:
Preliminary evidence from in vitro enzyme assays and computational modeling. No animal or human trials have been conducted with these specific peptides.
Study Age:
Published in 2024; represents current food-derived peptide research.
Original Title:
Screening and molecular dynamics simulation of ACE inhibitory tripeptides derived from milk fermented with Lactobacillus delbrueckii QS306.
Published In:
Food & function, 15(5), 2655-2667 (2024)
Database ID:
RPEP-09547

Evidence Hierarchy

Meta-Analysis / Systematic Review
Randomized Controlled Trial
Cohort / Case-Control
Cross-Sectional / ObservationalSnapshot without intervening
This study
Case Report / Animal Study
What do these levels mean? →

Frequently Asked Questions

Can drinking fermented milk actually lower blood pressure?

Some clinical studies have shown modest blood pressure reductions from fermented dairy, and this study identifies specific peptides that may be responsible. However, the amount of active peptide you get from a serving of yogurt or kefir hasn't been quantified in this research.

How do these peptides compare to ACE inhibitor medications?

ACE inhibitor drugs are far more potent and have decades of clinical evidence. These food-derived peptides are much weaker and would likely only be relevant as a complementary dietary strategy, not a pharmaceutical replacement.

Read More on RethinkPeptides

Cite This Study

RPEP-09547·https://rethinkpeptides.com/research/RPEP-09547

APA

Wu, Nan; Li, Puyu; Shuang, Quan; Wuhanqimuge. (2024). Screening and molecular dynamics simulation of ACE inhibitory tripeptides derived from milk fermented with Lactobacillus delbrueckii QS306.. Food & function, 15(5), 2655-2667. https://doi.org/10.1039/d3fo03320a

MLA

Wu, Nan, et al. "Screening and molecular dynamics simulation of ACE inhibitory tripeptides derived from milk fermented with Lactobacillus delbrueckii QS306.." Food & function, 2024. https://doi.org/10.1039/d3fo03320a

RethinkPeptides

RethinkPeptides Research Database. "Screening and molecular dynamics simulation of ACE inhibitor..." RPEP-09547. Retrieved from https://rethinkpeptides.com/research/wu-2024-screening-and-molecular-dynamics

Access the Original Study

Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.

This study breakdown was produced by the RethinkPeptides research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.