A Venomics Approach to the Identification and Characterization of Bioactive Peptides From Animal Venoms for Colorectal Cancer Therapy: Protocol for a Proof-of-Concept Study.

Shahzadi, Syeda Kiran et al.·JMIR research protocols·2021·Preliminary Evidenceother
RPEP-05753OtherPreliminary Evidence2021RETHINKTHC RESEARCH DATABASErethinkthc.com/research

Quick Facts

Study Type
other
Evidence
Preliminary Evidence
Sample
N=not applicable
Participants
Protocol for screening venom peptides against colorectal cancer cell lines (no results yet)

What This Study Found

Protocol describes high-throughput screening of 2,500 peptides from 20 animal venoms against colorectal cancer cell lines, with plans to isolate and characterize the most potent anti-CRC peptides.

Key Numbers

2,500 peptides; 20 venoms; 3 CRC cell lines; 2 control cell lines; MTT assay; top 3 venoms for peptide isolation

How They Did This

Proof-of-concept protocol. Will screen 2,500 peptides from 20 venoms using MTT cytotoxicity assay against 3 CRC cell lines and 2 control cell lines. Top 3 venoms will be further fractionated to identify specific anti-cancer peptides.

Why This Research Matters

Colorectal cancer is a leading cause of cancer death. Current treatments have significant side effects. Venom-derived peptides could provide targeted cancer-killing agents with different mechanisms than existing drugs.

What This Study Doesn't Tell Us

Protocol only. No results available. Cell line screening does not predict clinical efficacy. The specificity of venom peptides in living organisms may differ from cell culture results.

Trust & Context

Original Title:
A Venomics Approach to the Identification and Characterization of Bioactive Peptides From Animal Venoms for Colorectal Cancer Therapy: Protocol for a Proof-of-Concept Study.
Published In:
JMIR research protocols, 10(12), e31128 (2021)
Database ID:
RPEP-05753

Evidence Hierarchy

Meta-Analysis / Systematic Review
Randomized Controlled Trial
Cohort / Case-Control
Cross-Sectional / ObservationalSnapshot without intervening
This study
Case Report / Animal Study
What do these levels mean? →

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Cite This Study

RPEP-05753·https://rethinkpeptides.com/research/RPEP-05753

APA

Shahzadi, Syeda Kiran; Karuvantevida, Noushad; Banerjee, Yajnavalka. (2021). A Venomics Approach to the Identification and Characterization of Bioactive Peptides From Animal Venoms for Colorectal Cancer Therapy: Protocol for a Proof-of-Concept Study.. JMIR research protocols, 10(12), e31128. https://doi.org/10.2196/31128

MLA

Shahzadi, Syeda Kiran, et al. "A Venomics Approach to the Identification and Characterization of Bioactive Peptides From Animal Venoms for Colorectal Cancer Therapy: Protocol for a Proof-of-Concept Study.." JMIR research protocols, 2021. https://doi.org/10.2196/31128

RethinkPeptides

RethinkPeptides Research Database. "A Venomics Approach to the Identification and Characterizati..." RPEP-05753. Retrieved from https://rethinkpeptides.com/research/shahzadi-2021-a-venomics-approach-to

Access the Original Study

Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.

This study breakdown was produced by the RethinkPeptides research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.