Formulation of pH-responsive nanoplexes based on an antimicrobial peptide and sodium alginate for targeted delivery of vancomycin against resistant bacteria.

Shahin, Shourok et al.·Biological chemistry·2025·Preliminary Evidencelaboratory and animal study
RPEP-13536Laboratory and animal studyPreliminary Evidence2025RETHINKTHC RESEARCH DATABASErethinkthc.com/research

Quick Facts

Study Type
laboratory and animal study
Evidence
Preliminary Evidence
Sample
N=Not specified (mouse infection model)
Participants
Mice with MRSA systemic infection; S. aureus and MRSA in vitro

What This Study Found

pH-responsive nanoplexes combining a plant antimicrobial peptide with vancomycin showed 2-fold stronger activity against MRSA, 5-fold better biofilm eradication, and 4-5 fold reduced bacterial burden in kidneys, liver, and blood of mice with MRSA infection.

Key Numbers

Particle size 159.5 nm. Encapsulation efficiency 82.34%. 2-fold enhanced activity vs S. aureus and MRSA. 5-fold greater MRSA biofilm eradication. In vivo: 5-fold reduction in kidney MRSA, 4-fold in liver and blood. pH-accelerated VCM release at acidic pH.

How They Did This

Formulated nanoplexes from plant AMP and sodium alginate loaded with vancomycin. Characterized size, zeta potential, encapsulation, pH-responsive release. In vitro: MIC, biofilm eradication. In vivo: MRSA systemic infection in mice, organ bacterial burden, inflammation markers.

Why This Research Matters

MRSA is a leading cause of hospital deaths. This nanoparticle delivery system makes vancomycin far more effective by combining it with an antimicrobial peptide in a pH-triggered release system.

What This Study Doesn't Tell Us

Specific plant AMP not named in abstract. Mouse model may not reflect human MRSA infection. Toxicity profile needs more characterization. Manufacturing scalability not addressed.

Trust & Context

Original Title:
Formulation of pH-responsive nanoplexes based on an antimicrobial peptide and sodium alginate for targeted delivery of vancomycin against resistant bacteria.
Published In:
Biological chemistry, 406(8-9), 369-389 (2025)
Database ID:
RPEP-13536

Evidence Hierarchy

Meta-Analysis / Systematic Review
Randomized Controlled Trial
Cohort / Case-Control
Cross-Sectional / ObservationalSnapshot without intervening
This study
Case Report / Animal Study
What do these levels mean? →

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Cite This Study

RPEP-13536·https://rethinkpeptides.com/research/RPEP-13536

APA

Shahin, Shourok; Omolo, Calvin A; Elhassan, Eman; Ismail, Eman A; Farukh, Sania; Govender, Jasoda; Faya, Mbuso; Govender, Thirumala. (2025). Formulation of pH-responsive nanoplexes based on an antimicrobial peptide and sodium alginate for targeted delivery of vancomycin against resistant bacteria.. Biological chemistry, 406(8-9), 369-389. https://doi.org/10.1515/hsz-2025-0142

MLA

Shahin, Shourok, et al. "Formulation of pH-responsive nanoplexes based on an antimicrobial peptide and sodium alginate for targeted delivery of vancomycin against resistant bacteria.." Biological chemistry, 2025. https://doi.org/10.1515/hsz-2025-0142

RethinkPeptides

RethinkPeptides Research Database. "Formulation of pH-responsive nanoplexes based on an antimicr..." RPEP-13536. Retrieved from https://rethinkpeptides.com/research/shahin-2025-formulation-of-phresponsive-nanoplexes

Access the Original Study

Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.

This study breakdown was produced by the RethinkPeptides research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.