This companion paper provides validation data confirming that bradykinin and substance P protease activity assays work reliably with capillary blood and dried blood card samples.
Validated across 3 formatsAssay validation confirms reliability for serum, capillary blood, and dried blood cards
What the researchers found
Validation data confirms bradykinin and substance P protease activity assays are reliable when using capillary blood and blood card samples, supporting the protocols described in the companion methods paper.
Why it matters
Validation data is essential for researchers adopting new assay formats. Without it, labs cannot confidently use the simplified sample collection methods for protease activity measurement.
The numbers in context
2 assays validated for 2 alternative sample types
How the study worked
Validation experiments comparing bradykinin and substance P protease assay performance across serum, capillary blood, and dried blood card sample types. Statistical analysis of precision, accuracy, and reproducibility.
Who was studied
Laboratory validation study (no patient population)
What this study cannot tell us
Data paper — focused on assay validation rather than clinical application. Limited to the specific neuropeptide substrates tested.
How to read the evidence
Preliminary — method validation data supporting assay adoption but not a clinical study.
When this study was published
Published in 2020; provides foundation data for ongoing use of these assays.
The bigger picture
Data papers like this enable reproducibility and adoption of new methods across laboratories, which is critical for standardizing biomarker measurements in inflammation research.
Questions still open
- How stable are neuropeptide protease measurements on dried blood cards over different storage times?
- Can these validated methods be used in clinical diagnostic settings?
- What is the minimum blood volume needed for reliable results from capillary samples?
Common questions
Why is a separate validation paper needed?
What does protease activity tell researchers?
Read the original research
Validation data for the use of bradykinin and substance P protease activity assays with capillary blood and blood cards.
Data in brief, 28, 104873
Citation
Schreiber, Ulrich; Bayer, Malte; König, Simone. (2020). Validation data for the use of bradykinin and substance P protease activity assays with capillary blood and blood cards.. Data in brief, 28, 104873. https://doi.org/10.1016/j.dib.2019.104873