Validation Data for Bradykinin and Substance P Protease Assays Using Capillary Blood and Blood Cards
This companion paper provides validation data confirming that bradykinin and substance P protease activity assays work reliably with capillary blood and dried blood card samples.
Quick Facts
What This Study Found
Validation data confirms bradykinin and substance P protease activity assays are reliable when using capillary blood and blood card samples, supporting the protocols described in the companion methods paper.
Key Numbers
2 assays validated for 2 alternative sample types
How They Did This
Validation experiments comparing bradykinin and substance P protease assay performance across serum, capillary blood, and dried blood card sample types. Statistical analysis of precision, accuracy, and reproducibility.
Why This Research Matters
Validation data is essential for researchers adopting new assay formats. Without it, labs cannot confidently use the simplified sample collection methods for protease activity measurement.
The Bigger Picture
Data papers like this enable reproducibility and adoption of new methods across laboratories, which is critical for standardizing biomarker measurements in inflammation research.
What This Study Doesn't Tell Us
Data paper — focused on assay validation rather than clinical application. Limited to the specific neuropeptide substrates tested.
Questions This Raises
- ?How stable are neuropeptide protease measurements on dried blood cards over different storage times?
- ?Can these validated methods be used in clinical diagnostic settings?
- ?What is the minimum blood volume needed for reliable results from capillary samples?
Trust & Context
- Key Stat:
- Validated across 3 formats Assay validation confirms reliability for serum, capillary blood, and dried blood cards
- Evidence Grade:
- Preliminary — method validation data supporting assay adoption but not a clinical study.
- Study Age:
- Published in 2020; provides foundation data for ongoing use of these assays.
- Original Title:
- Validation data for the use of bradykinin and substance P protease activity assays with capillary blood and blood cards.
- Published In:
- Data in brief, 28, 104873 (2020)
- Authors:
- Schreiber, Ulrich, Bayer, Malte, König, Simone
- Database ID:
- RPEP-05118
Evidence Hierarchy
Frequently Asked Questions
Why is a separate validation paper needed?
The main methods paper describes how to run the assays, while this paper provides the statistical data proving they work correctly with different sample types. Labs need both to confidently adopt new methods.
What does protease activity tell researchers?
Proteases are enzymes that break down proteins and peptides. Their activity levels change in inflammation, pain, and disease. Measuring protease activity using neuropeptide reporters provides a window into these biological processes.
Read More on RethinkPeptides
Cite This Study
https://rethinkpeptides.com/research/RPEP-05118APA
Schreiber, Ulrich; Bayer, Malte; König, Simone. (2020). Validation data for the use of bradykinin and substance P protease activity assays with capillary blood and blood cards.. Data in brief, 28, 104873. https://doi.org/10.1016/j.dib.2019.104873
MLA
Schreiber, Ulrich, et al. "Validation data for the use of bradykinin and substance P protease activity assays with capillary blood and blood cards.." Data in brief, 2020. https://doi.org/10.1016/j.dib.2019.104873
RethinkPeptides
RethinkPeptides Research Database. "Validation data for the use of bradykinin and substance P pr..." RPEP-05118. Retrieved from https://rethinkpeptides.com/research/schreiber-2020-validation-data-for-the
Access the Original Study
Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.
This study breakdown was produced by the RethinkPeptides research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.