rethinkPeptides Search
Menu
Research citation

Impact of spin-freezing parameters and excipient composition on product stability of a PEGylated peptide formulation.

Laboratory StudyPreliminary evidence

This record provides bibliographic details and links to the original research. An editorial study breakdown is not available.

What the researchers found

For a PEGylated peptide drug, the choice of sugar excipient (trehalose, mannitol, or sucrose-mannitol) had a far greater impact on stability than spin-freezing parameters. Trehalose gave the best stability. Sucrose-mannitol degraded badly at high temperature.

Why it matters

Continuous freeze-drying is a promising manufacturing method for peptide drugs. This study shows that formulation choice matters more than processing conditions for product stability.

The numbers in context

Three formulations tested: trehalose, mannitol, sucrose-mannitol (75:25). Four spin-freezing conditions. Storage at 2-8°C or 50°C for up to 13 weeks. Sucrose-mannitol showed cake collapse, browning, and degradation at 50°C.

How the study worked

Compared trehalose, mannitol, and sucrose-mannitol formulations across four spin-freezing conditions. Evaluated peptide concentration, monomer content, and cake morphology after batch drying and accelerated/refrigerated storage.

Who was studied

PEGylated peptide formulations under various processing and storage conditions

What this study cannot tell us

Single PEGylated peptide studied. Results may not generalize to all peptide drugs. 50°C is an accelerated stress condition, not normal storage.

Read the original research

Impact of spin-freezing parameters and excipient composition on product stability of a PEGylated peptide formulation.

International journal of pharmaceutics, 683, 126007

Citation

Schaal, Zarah; Bockstal, Pieter-Jan Van; Lammens, Joris; Lenger, Julian H; Funke, Adrian P; Schneid, Stefan C; Beer, Thomas De. (2025). Impact of spin-freezing parameters and excipient composition on product stability of a PEGylated peptide formulation.. International journal of pharmaceutics, 683, 126007. https://doi.org/10.1016/j.ijpharm.2025.126007