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Study breakdown

How Enkephalin Peptides Slow Down the Colon — The Delta Receptor Mechanism

In VitroModerate evidence
The takeaway

Enkephalins inhibit relaxation nerve signals in human colon muscle through delta-opioid receptors, directly explaining one mechanism behind opioid-induced constipation.

Delta receptors: 100x more potent than mu

Delta-selective opioid agonists were 100-fold more effective than mu agonists at inhibiting colon relaxation nerve signals

What the researchers found

Enkephalins inhibit non-adrenergic, non-cholinergic inhibitory neurotransmission in human colon through prejunctional delta-opioid receptors with high selectivity.

Why it matters

This explains one mechanism by which opioids cause constipation. By blocking the nerve signals that normally relax the colon, opioid peptides reduce colon movement.

How the study worked

Sucrose-gap technique measured electrical responses in human colon circular muscle. Multiple opioid agonists and antagonists were tested to determine receptor selectivity.

What this study cannot tell us

In-vitro study using human tissue. The isolated tissue preparation removes normal neural network activity. Only one region of the colon was studied.

How to read the evidence

Moderate in-vitro study using human colon tissue with thorough pharmacological characterization. Directly relevant to human physiology.

When this study was published

Published in 1990. The delta-opioid mechanism of gut dysmotility has been validated and informs current approaches to managing opioid-induced constipation.

The bigger picture

This study identified the specific receptor mechanism behind opioid constipation in human tissue. By blocking the nerve signals that relax the colon, enkephalins effectively slow gut transit. This understanding has informed the development of peripherally-acting opioid antagonists (like methylnaltrexone) that treat opioid-induced constipation without affecting pain relief.

Questions still open

  • Could delta-selective opioid antagonists specifically treat constipation without affecting pain relief?
  • Are delta receptor levels altered in irritable bowel syndrome?

Common questions

How does this relate to opioid-induced constipation?
The colon needs inhibitory nerve signals to relax and move contents along. Enkephalins block these signals through delta receptors, preventing normal relaxation cycles. This slows transit and causes constipation — the same mechanism behind opioid medication side effects.
Can this mechanism be blocked without affecting pain relief?
Yes. Drugs like methylnaltrexone block opioid receptors in the gut without crossing into the brain, relieving constipation while preserving pain relief. This study helped identify the specific receptor target.

Read the original research

Enkephalins modulate inhibitory neuromuscular transmission in circular muscle of human colon via delta-opioid receptors.

The Journal of physiology, 431, 465-78

Citation

Hoyle, C H; Kamm, M A; Burnstock, G; Lennard-Jones, J E. (1990). Enkephalins modulate inhibitory neuromuscular transmission in circular muscle of human colon via delta-opioid receptors.. The Journal of physiology, 431, 465-78.