Regulation of Opioid Receptors by Their Endogenous Opioid Peptides.

Gupta, Achla et al.·Cellular and molecular neurobiology·2021·ModeratePreclinical Animal Study
RPEP-05429Preclinical Animal StudyModerate2021RETHINKTHC RESEARCH DATABASErethinkthc.com/research

Quick Facts

Study Type
Preclinical Animal Study
Evidence
Moderate
Sample
N=Animal study (both sexes, sizes not specified)
Participants
C57BL mice lacking proenkephalin, beta-endorphin, or both vs wild-type

What This Study Found

Loss of beta-endorphin and/or proenkephalin caused differential, region-specific, and sex-specific modulation of mu, delta, and kappa opioid receptor expression and activity, with no compensatory increase in dynorphin-derived peptides.

Key Numbers

3 knockout types; mu/delta/kappa all affected; region-specific; sex-specific; Leu-enkephalin mainly from proenkephalin; no dynorphin compensation

How They Did This

Knockout mouse study. Mice lacking proenkephalin, beta-endorphin, or both were compared to wild-type. Leu-enkephalin and dynorphin levels measured by mass spectrometry. Opioid receptor levels and G-protein activity measured in specific brain regions. Males and females compared.

Why This Research Matters

Opioid receptors are major drug targets for pain, addiction, and mood. Knowing that the body's own opioid peptides regulate these receptors helps explain individual differences in pain sensitivity and addiction vulnerability.

What This Study Doesn't Tell Us

Mouse knockout study. Complete absence of a peptide from birth does not mimic natural human variation. Compensatory developmental changes may mask acute effects. Cannot directly extrapolate sex differences to humans.

Trust & Context

Original Title:
Regulation of Opioid Receptors by Their Endogenous Opioid Peptides.
Published In:
Cellular and molecular neurobiology, 41(5), 1103-1118 (2021)
Database ID:
RPEP-05429

Evidence Hierarchy

Meta-Analysis / Systematic Review
Randomized Controlled Trial
Cohort / Case-Control
Cross-Sectional / ObservationalSnapshot without intervening
This study
Case Report / Animal Study
What do these levels mean? →

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Cite This Study

RPEP-05429·https://rethinkpeptides.com/research/RPEP-05429

APA

Gupta, Achla; Gullapalli, Srinivas; Pan, Hui; Ramos-Ortolaza, Dinah L; Hayward, Michael D; Low, Malcom J; Pintar, John E; Devi, Lakshmi A; Gomes, Ivone. (2021). Regulation of Opioid Receptors by Their Endogenous Opioid Peptides.. Cellular and molecular neurobiology, 41(5), 1103-1118. https://doi.org/10.1007/s10571-020-01015-w

MLA

Gupta, Achla, et al. "Regulation of Opioid Receptors by Their Endogenous Opioid Peptides.." Cellular and molecular neurobiology, 2021. https://doi.org/10.1007/s10571-020-01015-w

RethinkPeptides

RethinkPeptides Research Database. "Regulation of Opioid Receptors by Their Endogenous Opioid Pe..." RPEP-05429. Retrieved from https://rethinkpeptides.com/research/gupta-2021-regulation-of-opioid-receptors

Access the Original Study

Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.

This study breakdown was produced by the RethinkPeptides research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.