Collagen Peptides With Calcium and Vitamin D Improved Skin and Reduced Hair Loss in Menopausal Women

Six months of collagen peptide supplementation improved skin hydration by 23% and reduced hair shedding in menopausal women, but did not affect bone markers.

Duangjai, Acharaporn et al.·Clinics and practice·2025·Moderate Evidencerct
RPEP-10782RctModerate Evidence2025RETHINKTHC RESEARCH DATABASErethinkthc.com/research

Quick Facts

Study Type
rct
Evidence
Moderate Evidence
Sample
Menopausal women (number not specified in abstract)
Participants
Menopausal women (number not specified in abstract)

What This Study Found

Fish-derived collagen peptides combined with calcium and vitamin D3 significantly improved skin hydration by 23% and skin elasticity by 8.52% in menopausal women after six months. Collagen alone improved elasticity by 12.23%. All active supplement groups (calcium+D3, collagen, and the combination) significantly reduced hair shedding compared to placebo.

However, none of the supplement combinations produced significant changes in bone turnover markers (P1NP, BAP, osteocalcin) or body composition over the six-month period. Safety markers (creatinine, ALT, AST) remained normal across all groups.

Key Numbers

4 groups · 6 months · Skin hydration +23% (combo group) · Skin elasticity +8.52% (combo) and +12.23% (collagen alone) · Reduced hair shedding in all active groups · No bone marker changes

How They Did This

Randomized controlled trial with four groups: placebo, calcium 1000 mg + vitamin D3 400 IU, collagen peptides 5 g, and the combination of all three. Participants received daily supplementation for six months. Outcomes included body composition, bone turnover markers (P1NP, BAP, osteocalcin), skin hydration, skin elasticity, transepidermal water loss, hair loss, and safety labs.

Why This Research Matters

Menopause causes estrogen loss that simultaneously affects bones, skin, and hair. This study tests whether a combined supplement approach can address multiple symptoms at once. While the bone benefits weren't seen in this timeframe, the significant skin and hair improvements suggest collagen peptides offer real cosmetic benefits for menopausal women.

The Bigger Picture

Collagen peptide supplements have gained enormous consumer popularity, but clinical evidence has lagged behind marketing claims. This RCT adds to a growing body of evidence supporting collagen's benefits for skin — while importantly showing that bone benefits may require longer supplementation periods or different formulations.

What This Study Doesn't Tell Us

The sample size is not specified in the abstract, which makes it difficult to assess statistical power. Six months may be too short to detect changes in bone density or bone turnover markers. The study does not specify blinding procedures. Specific participant numbers per group are not reported.

Questions This Raises

  • ?Would a longer study period (12+ months) reveal bone density improvements from collagen and calcium/D3 supplementation?
  • ?How do fish-derived collagen peptides compare to bovine or marine collagen for skin and hair outcomes?
  • ?Are the skin hydration improvements maintained after supplementation is discontinued?

Trust & Context

Key Stat:
23% more hydrated Skin hydration improvement in menopausal women after 6 months of collagen + calcium + vitamin D supplementation
Evidence Grade:
This is a randomized controlled trial with a placebo group and multiple active comparators, supporting moderate evidence strength. However, the unspecified sample size and lack of detail on blinding procedures limit the certainty of the findings.
Study Age:
Published in 2025, this is very recent research addressing the growing consumer interest in collagen peptide supplementation with rigorous clinical trial methodology.
Original Title:
Calcium and Vitamin D Supplementation with and Without Collagen on Bone Density and Skin Elasticity in Menopausal Women-A Randomized Controlled Study.
Published In:
Clinics and practice, 15(9) (2025)
Database ID:
RPEP-10782

Evidence Hierarchy

Meta-Analysis / Systematic Review
Randomized Controlled Trial
Cohort / Case-Control
Cross-Sectional / ObservationalSnapshot without intervening
This study
Case Report / Animal Study
What do these levels mean? →

Frequently Asked Questions

Does collagen supplementation help with menopause-related skin changes?

This study suggests yes — menopausal women taking 5g of fish collagen peptides daily for 6 months saw significant improvements in skin hydration (23%) and elasticity (8–12%). The benefits were seen with collagen alone and were enhanced when combined with calcium and vitamin D.

Can collagen supplements improve bone health during menopause?

In this 6-month study, neither collagen peptides nor calcium and vitamin D supplementation changed bone turnover markers. Bone remodeling is a slow process, and longer supplementation periods may be needed to see skeletal benefits. The supplements were safe and did not negatively affect kidney or liver function.

Read More on RethinkPeptides

Related articles coming soon.

Cite This Study

RPEP-10782·https://rethinkpeptides.com/research/RPEP-10782

APA

Duangjai, Acharaporn; Srivilai, Jukkarin; Nangola, Sawitree; Amornlerdpison, Doungporn. (2025). Calcium and Vitamin D Supplementation with and Without Collagen on Bone Density and Skin Elasticity in Menopausal Women-A Randomized Controlled Study.. Clinics and practice, 15(9). https://doi.org/10.3390/clinpract15090168

MLA

Duangjai, Acharaporn, et al. "Calcium and Vitamin D Supplementation with and Without Collagen on Bone Density and Skin Elasticity in Menopausal Women-A Randomized Controlled Study.." Clinics and practice, 2025. https://doi.org/10.3390/clinpract15090168

RethinkPeptides

RethinkPeptides Research Database. "Calcium and Vitamin D Supplementation with and Without Colla..." RPEP-10782. Retrieved from https://rethinkpeptides.com/research/duangjai-2025-calcium-and-vitamin-d

Access the Original Study

Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.

This study breakdown was produced by the RethinkPeptides research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.