Temperature and ion dual responsive biphenyl-dipeptide supramolecular hydrogels as extracellular matrix mimic-scaffolds for cell culture applications.

Biphenylacetic acid-diphenylalanine (BPAA-FF) forms homogeneous, transparent hydrogels via temperature or ion triggers, which effectively support L929 cell adhesion and proliferation in both 2D and 3D cultures under physiological conditions.

The study synthesized several BPAA-dipeptide compounds using solid phase peptide synthesis and evaluated their gelation behavior under temperature changes and ion addition. Cell culture experiments with L929 fibroblasts assessed the hydrogels' ability to support cell growth.

Developing hydrogels that mimic the extracellular matrix and respond to physiological stimuli is crucial for creating better scaffolds for tissue engineering and regenerative therapies.

The study focuses on a limited set of dipeptides and cell types; further research is needed to confirm biocompatibility and functionality across diverse cells and in vivo models.